Glucoamylase GA-01L for brewery industry

 PRODUCT DESCRIPTION

The glucoamylase is produced by submerged fermentation of Aspergillus niger followed by purification and formulation. The product is used in starch sugar processing, alcohol fermentation, distilling, rice wine, vinegar, brewing, organic acid and antibiotics fermentation.

 MECHANISM

Glucoamylase breaks downα-1,4-glycosidic bond of starch from non-reducing end to produce glucose and can also hydrolyze α-1,6 glycosidic bond slowly. At the branch of α-1,6 bond, it splits off α-1.6 bonds first then α-1.4 bonds. Therefore, it can successively liberate glucose unit of amylose from the non-reducing end to produce glucose.

 REACTION PARAMETERS

PARAMETERS

RANGE

Activity Temperature

30℃-65℃

Optimum Temperature

55℃-62℃

Activity pH

3.5-6.0

4.0-5.0
PRODUCT SPECIFICATION

Items

Description

Declared Activity*

150,000 u/ml

Physical Form

Liquid

Color**

Brown

Odour

Normal microbial fermentation odour.

*Definition of Unit: 1 unit of Glucoamylase equals to the amount of enzyme which hydrolyzes soluble starch to get 1mg glucose at 40 and pH4.6 in 1h.
** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.

 PRODUCT STANDARD
The product complies with GB1886.174.

No.

ITEMS

INDEX

1

Bulk density/(g/ml)

1.05-1.20

2

pH/(25℃)

3.5–5.5

3

Lead/(mg/kg)

≤5.0

4

Arsenic/(mg/kg)

≤3.0

5

Total viable count/(CFU/mL)

≤50000

6

Coliform Bacteria/(CFU/mL)

≤30

7

Escherichia coli

(CFU/mL)

≤10

(MPN/mL)

≤3.0

8

Salmonella/(25mL)

Not Detected

 BENEFITS

lMaximizes conversion of starch substrates to fermentable sugars
lProduces highly attenuated beer in a cost-effective manner
lMaintain consistent fermentability, regardless of variations in raw material quality

RECOMMENDED DOSAGE

The recommended dosage is 0.4-1.0L of the enzyme preparation per ton of total raw materials, added at mashing-in. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.

 SAFE HANDLING PRECAUTIONS

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.

 WARNINGS

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.

 PACKAGE AND STORAGE

ØPackage25kgs/drum; 1,125kgs/drum.
ØStorage Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.
ØShelf life:  12 months in a dry and cool place.

 
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