Pullulanase for yield increase in brewing PL-01L

PRODUCT DESCRIPTION

This product is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It is a thermal stable debranching enzyme able to work at low pH, and is used in brewing, starch sugar processing, monosodium glutamate and alcohol fermentation, etc., to maximize sugar yield and the yield of final product.

 MECHANISM

Pullulanase is a type of isoamylase which can selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides, thereby, making the complete hydrolysis of branched starch possible. Together with other enzymes (glucoamylase, β-Amylase) it speeds up saccharification and increase glucose or maltose yield.

 REACTION PARAMETERS

PARAMETERS

RANGE

Activity Temperature

20-60

Optimum Temperature

45-52

Activity pH

3.0-6.8

3.0-5.0

 PRODUCT SPECIFICATION

Items

Description

Declared Activity*

2,000 u/ml

Physical Form

Liquid

Color**

Light brown

Odour

Normal microbial fermentation odour.

*Definition of Unit: 1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH5.8, 50.

** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.

 PRODUCT STANDARD
The product complies with GB1886.174.
No.
ITEMS
INDEX
1
Bulk density/(g/ml)
≤1.20
2
pH/(25℃)
3.0-6.0
3
Lead/(mg/kg)
≤5.0
4
Arsenic/(mg/kg)
≤3.0
5

Total viable count/(CFU/mL)

≤50000
6

Coliform Bacteria/(CFU/mL)

≤30
7

Escherichia coli

(CFU/mL)
<10
(MPN/mL)
≤3.0
8
Salmonella/(25mL)
Not Detected
 BENEFITS

lIncrease fermentable sugar;
lProvide a higher level of attenuation with low impact on yeast vitality;
lProduce highly attenuated beer in a cost-effective manner.

 APPLICATION RECOMMENDATION

The recommended dosage is 0.4-0.8L of the enzyme preparation per ton of total raw materials. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.

 SAFE HANDLING PRECAUTIONS

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.

 WARNINGS

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.

 PACKAGE AND STORAGE

ØPackage25kgs/drum; 1,125kgs/drum.
ØStorageKeep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.
ØShelf life:  12 months in a dry and cool place.

 

 

 

E-mail:
sunson@chinaenzymes.com
Tel:86-20-86586087
      86-10-58246891
Fax:86-20-86586895
      86-10-58246862
Please send email to us
SiteMap


Online customer service
Online customer service system