Pullulanase for yield increase in brewing PL-01L
PRODUCT DESCRIPTION
This product is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It is a thermal stable debranching enzyme able to work at low pH, and is used in brewing, starch sugar processing, monosodium glutamate and alcohol fermentation, etc., to maximize sugar yield and the yield of final product.
Pullulanase is a type of isoamylase which can selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides, thereby, making the complete hydrolysis of branched starch possible. Together with other enzymes (glucoamylase, β-Amylase) it speeds up saccharification and increase glucose or maltose yield.
PARAMETERS |
RANGE |
Activity Temperature |
20℃-60℃ |
Optimum Temperature |
45℃-52℃ |
Activity pH |
3.0-6.8
|
3.0-5.0 |
Items |
Description |
Declared Activity* |
2,000 u/ml
|
Physical Form |
Liquid |
Color** |
Light brown |
Odour |
Normal microbial fermentation odour. |
*Definition of Unit: 1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH5.8, 50℃.
** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.
No.
|
ITEMS
|
INDEX
|
|
1
|
Bulk density/(g/ml)
|
≤1.20
|
|
2
|
pH/(25℃)
|
3.0-6.0
|
|
3
|
Lead/(mg/kg)
|
≤5.0
|
|
4
|
Arsenic/(mg/kg)
|
≤3.0
|
|
5
|
Total viable count/(CFU/mL) |
≤50000
|
|
6
|
Coliform Bacteria/(CFU/mL) |
≤30
|
|
7
|
Escherichia coli |
(CFU/mL)
|
<10
|
(MPN/mL)
|
≤3.0
|
||
8
|
Salmonella/(25mL)
|
Not Detected
|
lIncrease fermentable sugar;
lProvide a higher level of attenuation with low impact on yeast vitality;
lProduce highly attenuated beer in a cost-effective manner.
The recommended dosage is 0.4-0.8L of the enzyme preparation per ton of total raw materials. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
ØPackage:25kgs/drum; 1,125kgs/drum.
ØStorage:Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.
ØShelf life: 12 months in a dry and cool place.
E-mail: sunson@chinaenzymes.com |
Tel:86-20-86586087 86-10-58246891 |
Fax:86-20-86586895 86-10-58246862 |