Brewing xylanase XY22L for xylan degradation in wort

 

PRODUCT DESCRIPTION  

Xylanase XY22L is produced by submerged fermentation of Trichoderma reesei followed by purification and formulation. Xylanase is able to degrade xylan in barley or malt to reduce wort viscosity and ease wort filtration. Therefore, the enzyme is widely used in brewing. 

MECHANISM 

As the main component of barley embryo cell wall, xylan is made up of xylose unit linked with β-1,4 glycosidic bond, besides the xylose residual there are many substitutions with big chemical groups along the backbone. The complete hydrolysis of xylan needs different enzymes to work synergistically, where β-1,4 -endo-xylanase plays a key role in degrading xylan into oligoxylans of different chain length and small amount of xylose. Xylan is the major responsible for wort viscosity, and degradation of the xylan leads to the great reduction of viscosity and easy wort filtration. 

REACTION PARAMETERS 

PARAMETERS

RANGE

Activity Temperature

40℃-80℃

Optimum Temperature

60℃-70℃

Activity pH

4.0-7.0

Optimum pH

4.2-5.5

 PRODUCT SPECIFICATION 

Items

Description

Declared Activity*

22,000u/ml

Physical Form

Liquid

Color**

Brown

Odour

Normal microbial fermentation odour.

 *Definition of Unit: 1 unit of Xylanase equals to the amount of enzyme, which hydrolyzes xylan to get 1 μmol of reducing sugar (Calculated as xylose) in 1 min at 50 and pH5.0. 

** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity. 

PRODUCT STANDARD  

The product complies with GB1886.174. 

No.

ITEMS

INDEX

1

Bulk density/(g/ml)

1.10-1.25

2

pH/(25)

4.0-6.0

3

Lead/(mg/kg)

5.0

4

Arsenic/(mg/kg)

3.0

5

Total viable count/(CFU/mL)

50,000

6

Coliform Bacteria/(CFU/mL)

30

7

Escherichia coli

(CFU/mL)

<10

(MPN/mL)

3.0

8

Salmonella/(25mL)

Not Detected

APPLICATION RECOMMENDATION 

The recommended dosage is 0.1-0.3L of the enzyme preparation per ton of total raw materials, added at mashing-in. However, the dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.

SAFE HANDLING PRECAUTIONS 

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor. 

WARNINGS 

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.

PACKAGE AND STORAGE

 Ø  Package 25kgs/drum; 1,125kgs/drum.

 Ø  Storage Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.

Ø  Shelf life:  12 months in a dry and cool place.

 

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