Food grade laccase enzyme with CAS 80498
The laccase is produced by submerged fermentation of Aspergillus oryzae followed by purification and formulation. Laccase catalyzes the oxidation of phenols and their derivatives, aromatic amines and their derivatives carboxylic acids and their derivatives, steroid hormones, metalloorganic compounds and some non-phenolic compounds, and the higher the redox potential of laccase, the wider the range of degradable substrates for it.
MECHANISM
Laccase is a polyphenol oxidase (ρ-dihydric phenol oxidase, EC1.10.3.2) containing four copper ions, belonging to the copper blue oxidase, present as a monomeric glycoprotein. Laccase exists in fungi, bacteria and plants. It mainly takes effect on phenol-like compounds, performing one-electron oxidation. It is capable of catalyzing the ring cleavage reaction of aromatic compounds. In industry, laccase can be paired with an electron mediator to facilitate electron transfer to a solid electrode wire. It can also be applied to textile dyeing/textile finishing, wine cork making, teeth whitening with many other industrial, environmental, diagnostic, and synthetic uses. Laccase can be used to remove the polyphenols as well as oxygen wherever necessary.
REACTION PARAMETERS
|
CONDITION |
RANGE |
|
Activity Temperature |
20℃-60℃ |
|
Optimum Temperature |
45℃-55℃ |
|
Activity pH |
3.5-6.0 |
|
Optimum pH |
4.0-5.0 |
PRODUCT STANDARD
The product complies with GB1886.174.
|
No. |
ITEMS |
INDEX |
|
|
1 |
Bulk density/(g/ml) |
1.05-1.20 |
|
|
2 |
pH/(25℃) |
3.5–5.5 |
|
|
3 |
Lead/(mg/kg) |
≤5.0 |
|
|
4 |
Arsenic/(mg/kg) |
≤3.0 |
|
|
5 |
Total viable count/(CFU/mL) |
≤50000 |
|
|
6 |
Coliform Bacteria/(CFU/mL) |
≤30 |
|
|
7 |
Escherichia coli |
(CFU/mL) |
<10 |
|
(MPN/mL) |
≤3.0 |
||
|
8 |
Salmonella/(25mL) |
Not Detected |
|
PRODUCT SPECIFICATION
Light brown liquid. Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.
APPLICATION RECOMMENDATION
The recommended dosage is 5-500g/t DS. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
WARNINGS
PACKAGE AND STORAGE
Package： 25kg/drum.
Storage： Keep sealed in a dry and cool place and avoid direct sunlight.
Shelf life: 12 months in a dry and cool place.
| E-mail: sunson@chinaenzymes.com |
| Tel:86-20-86586087 86-10-58246891 |
| Fax:86-20-86586895 86-10-58246862 |
