Food grade lysophospholipase enzymes for food additive
The Lysophospholipase is produced by submerged fermentation of Aspergillus niger followed by purification, formulation and drying. The product has high hydrolytic activity within a wide range of temperature and pH.
MECHANISM
Phospholipids are lipids containing phosphate group and can be divided into sphingolipids and glycerophospholipids according to their chemical structure. In plants phospholipids exist predominantly in the form of glycerophospholipids. The general formula of their structures is shown in figure 1. Depending on the type of hydrolysis they catalyze, phospholipases are categorized into phospholipase A1, phospholipase A2, phospholipase B, phospholipase C and phospholipase D as illustrated in the below figure. The present product belongs to phospholipase B.
Fig. 1 Specificity of different phospholipid enzyme
REACTION PARAMETERS
|
CONDITION |
RANGE |
|
Activity Temperature |
30℃-55℃ |
|
Optimum Temperature |
30℃-45℃ |
|
Activity pH |
4.0-8.0 |
|
Optimum pH |
5.5-7.5 |
White to gray-white powder. Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.
PRODUCT STANDARD
The product complies with GB1886.174.
|
No. |
ITEMS |
INDEX |
|
|
1 |
Particle size (%<40 mesh) |
≥80 |
|
|
2 |
≤8.0 |
||
|
3 |
Lead/(mg/kg) |
≤5.0 |
|
|
4 |
Arsenic/(mg/kg) |
≤3.0 |
|
|
5 |
Total viable count/(CFU/g) |
≤50000 |
|
|
6 |
Coliform Bacteria/(CFU/g) |
≤30 |
|
|
7 |
Escherichia coli |
(CFU/g) |
<10 |
|
(MPN/g) |
≤3.0 |
||
|
8 |
Salmonella/(25g) |
Not Detected |
|
APPLICATION RECOMMENDATION
The recommended dosage is 0.1-5kg/t DS. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
SAFE HANDLING PRECAUTIONS
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
WARNINGS
PACKAGE AND STORAGE
Ø Package： 25kg/drum.
Ø Storage： Keep sealed in a dry and cool place and avoid direct sunlight.
Ø Shelf life: 12 months in a dry and cool place.
| E-mail: sunson@chinaenzymes.com |
| Tel:86-20-86586087 86-10-58246891 |
| Fax:86-20-86586895 86-10-58246862 |
