Liquid pullulanase for starch debranching and complete starch hydrolysis

 

PRODUCT DESCRIPTION

 

This product is produced by submerged fermentation of Bacillus Licheniformis followed by purification and formulation. It is a thermal stable debranching enzyme able to work at low pH, and is widely used in brewing, starch sugar processing, monosodium glutamate and alcohol fermentation, etc.

 

MECHANISM

Pullulanase is a type of isoamylase which can selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides, thereby, making the complete hydrolysis of branched starch possible. Together with other enzymes (glucoamylase, β-Amylase) it speeds up saccharification and increase glucose or maltose yield.  

 

REACTION PARAMETERS

CONDITION	RANGE
Activity Temperature	40℃-65℃
Optimum Temperature	50℃-60℃
Activity pH	3.5-6.0
Optimum pH	4.0-5.0

 

PRODUCT SPECIFICATION

Brown liquid. Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.

 

PRODUCT STANDARD

The product complies with GB1886.174.

No.	ITEMS		INDEX
1	Bulk density/(g/ml)		1.05-1.20
2	pH/(25℃)		4.0 - 6.0
3	Lead/(mg/kg)		≤5.0
4	Arsenic/(mg/kg)		≤3.0
5	Total viable count/(CFU/mL)		≤50000
6	Coliform Bacteria/(CFU/mL)		≤30
7	Escherichia coli	(CFU/mL)	<10
		(MPN/mL)	≤3.0
8	Salmonella/(25mL)		Not Detected

 

APPLICATION RECOMMENDATION

The recommended dosage is 0.05-2kg/t DS. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with convenient volume.

 

SAFE HANDLING PRECAUTIONS

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.

 

WARNINGS

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.

 

 

PACKAGE AND STORAGE

Ø  Package： 25kg/Jerry can.

Ø  Storage： Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.

Ø  Shelf life:  12 months in a dry and cool place.