Glucose Isomerase for high fructose syrup
DESCRIPTION
Glucose isomerase (GI, EC 5.3.1.5; also known as D-xylose ketol isomerase, xylose isomerase (XI), xylose ketoisomerase, and xylose ketol-isomerase), is one of the three most commonly produced industrial enzymes, along with amylase and protease. This enzyme is extensively used in the industrial production of high-fructose corn syrup (HFCS), and it can also be used to boost cellulosic ethanol yield and used in the production of low glycemic index foods.
MECHANISM
The catalytic reaction of GI occurs in three major steps: (i) ring opening, (ii) isomerization, and (iii) ring closure as illustrated by the following.
GI requires divalent cations as cofactors for its isomerization activity. Typically, GI is known to exhibit the highest activity in the presence of divalent metal ions such as Mg2+, Mn2+ and Co2+; however, the specific metal ion requirement can vary depending on the source of the enzyme.
PRODUCT SPECIFICATION
Items |
Description |
Physical Form |
Powder |
Color** |
White |
Odour |
Normal microbial fermentation odour. |
** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.
PRODUCT STANDARD
The product complies with GB1886.174.
No. |
ITEMS |
INDEX |
|
1 |
Particle size (%<40 mesh) |
≥80 |
|
2 |
≤8.0 |
||
3 |
Lead/(mg/kg) |
≤5.0 |
|
4 |
Arsenic/(mg/kg) |
≤3.0 |
|
5 |
Total viable count/(CFU/g) |
≤50000 |
|
6 |
Coliform Bacteria/(CFU/g) |
≤30 |
|
7 |
Escherichia coli |
(CFU/g) |
<10 |
(MPN/g) |
≤3.0 |
||
8 |
Salmonella/(25g) |
Not Detected |
APPLICATIONS
l High-Fructose Corn Syrup (HFCS).
l Boost ethanol production by isomerization of xylose to xylulose that can be fermented to ethanol by yeast.
l Produce low glycemic index by converting glucose into fructose.
APPLICATION RECOMMENDATION
The highest activity required in the reaction system is Mg2+ and Co2+. Their optimum concentrations are 10-2mol/L and 10-3mol/L, respectively.
The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
SAFE HANDLING PRECAUTIONS
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
WARNINGS
PACKAGE AND STORAGE
- Package: 25kgs/drum; 1,125kgs/drum.
- Storage: Keep sealed in a dry and cool place and avoid direct sunlight.
- Shelf life: 12 months in a dry and cool place.
E-mail: sunson@chinaenzymes.com |
Tel:86-20-86586087 86-10-58246891 |
Fax:86-20-86586895 86-10-58246862 |